Domains in Tropoelastin That Mediate Elastin Deposition in Vitro and in Vivo -- Kozel et al. 278 (20): 18491 -- Journal of Biological Chemistry
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منابع مشابه
Domains in tropoelastin that mediate elastin deposition in vitro and in vivo.
Elastic fiber assembly is a complicated process involving multiple different proteins and enzyme activities. However, the specific protein-protein interactions that facilitate elastin polymerization have not been defined. To identify domains in the tropoelastin molecule important for the assembly process, we utilized an in vitro assembly model to map sequences within tropoelastin that facilitat...
متن کاملEvidence that tropoelastin is the primary precursor in elastin biosynthesis.
Tropoelastin, a 70,000-dalton protein, appears to be a soluble intermediate in the biosynthesis of insoluble elastin. Recently, Foster et al. (Foster, J. A., Mecham, R. P., Rich, C. B., Cronin, M. F., Levine, A., Imberman, M., and Salcedo, L. L. (1978) J. Biol. Chem 253, 27972803) have presented evidence for the existence of a 120,000to 130,000-dalton intermediate designated proelastin. This p...
متن کاملGlycosaminoglycans mediate the coacervation of human tropoelastin through dominant charge interactions involving lysine side chains.
Following cellular secretion into the extracellular matrix, tropoelastin is transported, deposited, and cross-linked to make elastin. Assembly by coacervation was examined for an isoform of tropoelastin that lacks the hydrophilic domain encoded by exon 26A. It is equivalent to a naturally secreted form of tropoelastin and shows similar coacervation performance to its partner containing 26A, the...
متن کاملTropoelastin interacts with cell-surface glycosaminoglycans via its COOH-terminal domain.
Using a biochemical and cell biological approach, we have identified a cell interaction site at the carboxyl terminus of tropoelastin. Cell interactions with the COOH-terminal sequence are not through the elastin-binding protein (EBP67) because neither VGVAPG-like peptides nor galactoside sugars altered adhesion. Our results also show that cell adhesion to tropoelastin is not promoted by integr...
متن کاملFunctional domains on elastin and microfibril-associated glycoprotein involved in elastic fibre assembly.
Studies in vitro suggest that the C-terminus of tropoelastin mediates elastin polymerization through an interaction with microfibril-associated proteins. In this study we have used cultured auricular chondrocytes as a model system to examine whether this interaction is critical for elastic fibre formation in vivo. Auricular chondrocytes, which deposit an abundant elastic fibre matrix, were cult...
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تاریخ انتشار 2006